Effects of energetic restriction diet on butyrylcholinesterase in obese women from southern Brazil - A longitudinal study

Objective Butyrylcholinesterase (BChE) activity has been associated with obesity, lipid concentrations, and CHE2 locus phenotypes. This, the aim of this study was to evaluate the effects of an energetic restriction diet intervention on anthropometrical and biochemical variables and on absolute and relative BChE activity in CHE2 C5+ and CHE2 C5- individuals. Subjects and methods One hundred eleven premenopausal obese women from Southern Brazil participated in an energetic restriction diet intervention (deficit of 2500 kJ/day) for 8 weeks. Their anthropometric and biochemical parameters were evaluated before and after the intervention. Plasma BChE activity was measured, and BChE bands in plasma and CHE2 locus phenotypes were detected by electrophoresis. Results The dietetic intervention decreased anthropometric and biochemical parameters as well as absolute BChE activity and relative activity of the G4 band. The CHE2 C5+ phenotype presented a different effect when compared with the CHE2 C5- phenotype. The CHE2 C5+ phenotype showed an effect in absolute BChE activity and in the relative activity of the G4 form, maintaining higher BChE activity regardless of the metabolic changes. Conclusion In our study, 8 weeks was not sufficient time to lower the body mass index to normal, but it was enough to significantly reduce the absolute BChE activity, which became similar to the levels in nonobese individuals. CHE2 C5+ individuals were resistant to the decrease in BChE activity compared to CHE2 C5- individuals. This shows that the diet did not affect the CHE2 and G4 fraction complex and that the products of the CHE2 locus in association with BChE have a role in energy metabolism, maintaining high levels of enzymatic activity even after dietary intervention.

Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women

Abstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms.

Zinco no plasma e eritrócito de atletas profissionais de uma equipe feminina brasileira de voleibol / Plasma and erythrocyte zinc in Brazilian professional female volleyball players

OBJETIVO: Avaliar o estado nutricional relativo ao zinco de 12 atletas profissionais do voleibol feminino. MÉTODOS: Determinou-se a concentração de zinco plasmático e eritrocitário por espectrofotometria de absorção atômica, e avaliou-se a ingestão dietética por meio de registro alimentar de três dias, de 12 atletas profissionais do sexo feminino, integrantes de uma equipe de voleibol de Curitiba, PR, na faixa etária de 18 a 24 anos, no ano de 2003. RESULTADOS: Todas as atletas apresentaram valores normais de zinco plasmático. Quanto ao zinco eritrocitário, 100,00 por cento apresentaram valores abaixo da normalidade. Em relação à ingestão dietética de zinco, 83,33 por cento apresentaram adequação. Decréscimos no conteúdo de zinco eritrocitário podem representar o efeito agudo de exercícios prolongados pela utilização enzimática da superóxido dismutase ou anidrase carbônica. CONCLUSÃO: O estado nutricional das atletas relativo ao zinco parece estar inadequado, pois, apesar dos valores normais para a concentração de zinco plasmático, a concentração eritrocitária esteve abaixo da faixa de normalidade para todas as atletas.

OBJECTIVE: This study aimed to evaluate the nutritional status of zinc in 12 female volleyball players. METHODS: Zinc levels in plasma and erythrocytes were assessed by atomic absorption spectrophotometry. Zinc intake of 12 professional female athletes of a volleyball team from Curitiba, PR, aging from 18 to 24 years in 2003, was determined by a 3-day food record. RESULTS: All athletes presented normal plasma zinc values. Erythrocyte zinc was lower than normal in 100 percent of the athletes. Dietary intake of zinc was adequate in 83.33 percent. The significant decreases in erythrocyte content could represent the acute effects of prolonged exercises which increases superoxide dismutase or carbonic anhydrase utilization. CONCLUSION: The athletes' zinc nutritional status seemed to be inadequate. They had normal plasma zinc levels but the erythrocyte zinc was below the normal values for all of them.